Abstract:
The aim of this study was to prepare an easily screening test kit for urinary cotinine assay.The semi-quantitative assay kit is provided for screening cotinine test in the field works. This method was based on Direct Barbituric acid (DBA) by using dropper instead of automatic pipette. The intensity of color reaction was compared with color-printed standard cotinine and graded as 1+, 2+, 3+ and 4+ with the cotinine concentration of 10, 25, 50, 100 μmol/L, respectively. Urine cotinine and creatinine was reported was CCR in nonsmoker(n=30), smoker(n=30) and high school male students (n=200). The results showed that the stability of urinary cotinine at least 8 days kept at -20oc.The percentage of error by using dropper of 100, 250, 500 μl was not significantly different from those of using automatic pipette (p>0.05). Within run occasionally smoker, and regularly smoker, respectively. Between run assay showed %CV of 8.60, 2.72, and 1.23 . By using quantitative method cotinine level 3.28 μmol/L in non smokers, and 55.10 μmol/L in smokers. The results impired that using cotinine assay kit can be distinguished smoker from non smoker group. To screening smoking status, questionnaire was performed in 200 participants. The results demonstrated that 33 of 34 persons. Showed positive for cotinine assay and 97 persons showed negative results for 100 passive smokers. The test kit showed high sensitivity of 97.06 %, high specificity of 96.39 %, positive predictive value for 84.62% and negative predictive value for 99.38%. As the results showed above, also the simplicity, convenienty and short-time consuming of the test. The kit can be used or screening test for urinary cotinue assay in the filed work.
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